Splet05. mar. 2024 · Buffers and MgCl 2 in PCR reactions. A typical reaction buffer for PCR would something like: 10 mM Tris, pH 8.3; 50 mM KCl; 1.5 mM MgCl 2; 0.01% gelatin; The MgCl 2 concentration in the final reaction mixture is usually between 0.5 to 5.0 mM, and the optimum concentration is determined empirically (typically between 1.0 - 1.5 mM). Mg 2+ … SpletWe would like to show you a description here but the site won’t allow us.
Mikrobiologie - chemlab
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